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polY1 [2019-09-17 10:19:37]
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polY1 [2019-09-17 10:19:37]

translesion synthesis (TLS-) DNA polymerase Y1, important for stationary phase mutagenesis, promotes efficient replisome progression through lagging-strand genes, thereby reducing potentially detrimental breaks and single-stranded DNA at these loci
locus
BSU_23870
pI
9.95
mw
46.86 kDa
protein length
414 aa Sequence Blast
gene length
1245 bp Sequence Blast
function
generation of mutations in stationary phase
product
translesion synthesis (TLS-) DNA polymerase Y1
essential
no
ec
2.7.7.7
synonyms
yqjH
Outlinks
KEGGBsubCycSubtiListUniProtExpression Browser

Genomic Context

      

categories

  • [category|SW 3|Information processing] → [category|SW 3.1|Genetics] → [category|SW 3.1.5|DNA repair/ recombination]
  • [category|SW 3|Information processing] → [category|SW 3.1|Genetics] → [category|SW 3.1.5|DNA repair/ recombination] → [category|SW 3.1.5.6|Other proteins]

    • Gene

    Coordinates
    2,482,269 → 2,483,513

    Phenotypes of a mutant

  • reduced UV-induced mutagenesis [Pubmed|12644484]
  • sensitive to blue light-induced DNA damage [pubmed|30054368]
  • increased sensitivity to Cr(VI) [pubmed|30745368]

    • The protein

    Catalyzed reaction/ biological activity

  • 2'-deoxyribonucleoside 5'-triphosphate + DNA(n) --> diphosphate + DNA(n+1) (according to UniProt)
  • required for Cr(VI)-induced mutagenesis [pubmed|30745368]

    • Protein family

  • [SW|DNA polymerase type-Y family] (according to UniProt)

    • Paralogous protein(s)

  • [protein|F0BC150F59730E64BA9AB3F979544EF6B5CC4E9E|PolY2], [protein|BE8D5E038C0FD748C0F9C3F18DCA601BFFE8EA52|UvrX]

    • Structure

  • [PDB|4IRC] (from E. coli, 35% identity) [pubmed|23525461]

    • [SW|Localization]

  • cytoplasm (homogeneous)

    • Expression and Regulation

    Operons

    genes
    [gene|C87D33852F263A553CA747608E6200E4AD8344B5|polY1]-[gene|61B7C51EB7E74226890010B61D8E41C02189A453|gndA]
    description
    [pubmed|22383849]

    regulation

  • constitutively expressed [Pubmed|15469515]
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    Biological materials

    Mutant

  • MGNA-C390 (yqjH::erm), available at the [https://shigen.nig.ac.jp/bsub/resource/strainGeneDisrupted/detail/2388 NBRP B. subtilis, Japan]
  • GP1111 (spc), available in [SW|Stülke] lab
  • BKE23870 (Δ[gene|C87D33852F263A553CA747608E6200E4AD8344B5|polY1]::erm trpC2) available at [http://www.bgsc.org/getdetail.php?bgscid=BKE23870 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CATAAACAAATCTCCTTTTC, downstream forward: _UP4_TGAATCGCTTGAAAAAAAGG
  • BKK23870 (Δ[gene|C87D33852F263A553CA747608E6200E4AD8344B5|polY1]::kan trpC2) available at [http://www.bgsc.org/getdetail.php?bgscid=BKK23870 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CATAAACAAATCTCCTTTTC, downstream forward: _UP4_TGAATCGCTTGAAAAAAAGG

    • References

    Reviews

  • 22933559,29856930

    • Original publications

  • 12644484,15469515,16045613,19924481,23686288,25713353,30054368,30096406,23525461,30745368,30916324